FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_74.3510000005f7b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_74.3510000005f7b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44027
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTGTGTCCTGTCTCTTATACAC4541.0311854089535966No Hit
ACTGTCTCTGTCTCTTATACACATCTC4491.0198287414541076No Hit
ACTGTCTGTGTCCTGTCTCTTCTACAC1800.4088400299816022No Hit
ACTGTCTCTGTCTCTTATACCCATCTC640.14536534399345855No Hit
ACTGTCTGTGTCTGTCTCTTATACACA470.10675267449519613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCT609.767973E-1021.02161420
ACATCTC609.767973E-1021.02161421
TTGTTTT900.020.99772818
TGGTTTT1100.020.99772818
TCCTTTT456.109494E-720.99772818
GGTGATT250.003331168620.99772816
ACTGTGT701.4551915E-1120.9977285
GTCACTG250.003331168620.99772810
GGCATTT250.003331168620.99772817
AGCTTTT558.416464E-920.99772818
GTGTCCG2550.020.9977288
GTGTCAT1850.020.9977288
GTGTCAC2050.020.9977288
GTCATGC250.003331168620.99772810
GTCACAT250.003331168620.99772810
TCTAGGG250.003331168620.99772811
AAATTTT507.163726E-820.99772818
GCGATTT354.4785746E-520.99772817
AATTTTT2650.020.99772819
ACGTTTT507.163726E-820.99772818
Produced by FastQC (version 0.11.2)