FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_72.3510000005f77e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_72.3510000005f77e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29812
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGAGGCTGTCTCTTATAC2400.8050449483429492No Hit
GTGTAGTGTGGCTGTCTCTTATACACA2180.7312491614115122No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC940.31530927143432175No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC850.28512008587146115No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC830.2784113779686032No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC670.22474171474573998No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC670.22474171474573998No Hit
GTGTAGTGTGGACCTGTCTCTTATACA620.20796994498859517No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC550.18448946732859253No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA480.16100898966858984No Hit
GTGTAGTGTGGAATCTGTCTCTTATAC430.14423721991144506No Hit
GTGTAGTGTGGATCTGTCTCTTATACA370.12411109620287133No Hit
GTGTAGTGTGGATTCTGTCTCTTATAC340.11404803434858447No Hit
GTGTAGTGTGGAGGGGCTGTCTCTTAT330.1106936803971555No Hit
GTGTAGTGTGGAACCTGTCTCTTATAC300.10063061854286864No Hit
GTGTAGTGTGGCTGTCTCTTCTACACA300.10063061854286864No Hit
GTGTAGTGTGGATACTGTCTCTTATAC300.10063061854286864No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT751.8189894E-1221.00000218
TGATTTT558.132702E-921.00000218
TGGAGAG751.8189894E-1221.0000029
CCTTTTT558.132702E-921.00000219
TGGATTG558.132702E-921.0000029
TGGATAG558.132702E-921.0000029
AGGTTTT751.8189894E-1221.00000218
GGGGTTT558.132702E-921.00000217
TGGAAAG558.132702E-921.0000029
GTGGATA950.021.0000028
GTGGACC1100.021.0000028
GGTGTTT751.8189894E-1221.00000217
GGAGATG250.003304153721.010
GAGGTGG455.967213E-721.011
AGGGGGG250.003304153721.012
AGGGGGC250.003304153721.012
GGAGAGG506.9610905E-821.010
GTGTTTT1300.021.018
GGGTTTT1300.021.018
GCCTTTT354.4125678E-521.018
Produced by FastQC (version 0.11.2)