FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_7.3510000005ef50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_7.3510000005ef50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57979
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC1640.2828610358923058No Hit
CTTCAGCAATGCGGGGGGGGTTTTTTT750.1293571810483106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATTT558.547431E-921.00000217
AGCTTTT651.200533E-1021.00000218
AAATTTT405.265769E-621.00000218
CAGCAAT53450.021.0000024
GATATTT405.265769E-621.00000217
TGCGCGG800.021.00000210
TGCGCGA558.547431E-921.00000210
ACGTTTT800.021.00000218
TGCGTGT558.547431E-921.00000210
TGCGTCT405.265769E-621.00000210
TAAGCAA558.547431E-921.0000023
TGCTGTG651.200533E-1021.00000210
TGCGAGC405.265769E-621.00000210
TGCTGGG800.021.00000210
GGTTCTG405.265769E-621.00000219
CCGTTTT651.200533E-1021.00000218
ATGCGCA651.200533E-1021.0000029
GCGTGTG405.265769E-621.00000211
GGGAGTT405.265769E-621.00000216
TCAGCAA49000.021.0000023
Produced by FastQC (version 0.11.2)