FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_69.3510000005f72e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_69.3510000005f72e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90287
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGAACTGGGGCTGTCTCTTATAC1480.16392171630467287No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTCTG354.507533E-521.02107620
AACTGGG33500.020.9977867
CTGGGGA1650.020.9977869
CACTGTC751.8189894E-1220.99778619
CCTTGAA83100.020.9977862
TAGTTTT1300.020.99778618
CTGGCTC1350.020.9977869
CTGGCGC1650.020.9977869
CTGGCGA1350.020.9977869
CTGGCCG1650.020.9977869
CTGGTCG1350.020.9977869
CTGGCAC651.2187229E-1020.9977869
CTGGTAA751.8189894E-1220.9977869
CTGGAGC1350.020.9977869
CTGGACG1300.020.9977869
TTGGTTT651.2187229E-1020.99778617
CAGGTTT651.2187229E-1020.99778617
TTGAACT89200.020.9977864
TGGGACG751.8189894E-1220.99778610
TGGGAAC651.2187229E-1020.99778610
Produced by FastQC (version 0.11.2)