FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_66.3510000005f6b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_66.3510000005f6b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34679
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATACCTGTCTCTTATACACA1270.36621586550938606No Hit
GCATTGGATACCCTGTCTCTTATACAC1050.3027768966809885No Hit
GCATTGGATACCGGCTGTCTCTTATAC920.2652902332823899No Hit
GCATTGGATACCGCCTGTCTCTTATAC740.21338562242279188No Hit
GCATTGGATACCAGCTGTCTCTTATAC650.18743331699299287No Hit
GCATTGGATACCGTCTGTCTCTTATAC580.16724819054759363No Hit
GCATTGGATACCTGCTGTCTCTTATAC570.16436460105539377No Hit
GCATTGGATACCACCTGTCTCTTATAC510.1470630641021944No Hit
GCATTGGATACCATCTGTCTCTTATAC490.14129588511779462No Hit
GCATTGGATACCCCTGTCTCTTATACA430.12399434816459529No Hit
GCATTGGATACCCGCTGTCTCTTATAC430.12399434816459529No Hit
GCATTGGATACCCTGTCTCTTCTACAC400.1153435796879956No Hit
GCATTGGATACCGACTGTCTCTTATAC380.10957640070359584No Hit
GCATTGGATACCCCCTGTCTCTTATAC370.10669281121139594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGA303.8285018E-421.00000210
ACCGGCT303.8285018E-421.00000210
ACCGGAG303.8285018E-421.00000210
ACCGGAC303.8285018E-421.00000210
AGCTTTT303.8285018E-421.00000218
AAATTTT303.8285018E-421.00000218
TCGGTTT303.8285018E-421.00000217
ATGGTTT303.8285018E-421.00000217
ACCGTGA303.8285018E-421.00000210
ACCGCCG303.8285018E-421.00000210
ACCGTAT303.8285018E-421.00000210
ACTTTTT1200.021.00000219
ACCTGTG303.8285018E-421.00000210
TACCGAA609.677024E-1021.0000029
ACCCCCG303.8285018E-421.00000210
GAATTTT609.677024E-1021.00000218
TACCCTA303.8285018E-421.0000029
CCGGGGC303.8285018E-421.00000211
TACCTGT1200.021.0000029
TACCCCG303.8285018E-421.0000029
Produced by FastQC (version 0.11.2)