FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_62.3510000005f63c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_62.3510000005f63c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75217
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTCTGTTGCTGTCTCTTATACACA6080.8083279045960354No Hit
ATGCTCTGTTGCGGCTGTCTCTTATAC1400.1861281359267187No Hit
ATGCTCTGTTGCCTGTCTCTTATACAC1300.17283326907481023No Hit
ATGCTCTGTTGCGGGGGGGGTTTTTTT970.12896020846351225No Hit
ATGCTCTGTTGCGGGGCTGTCTCTTAT930.12364226172274885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT2600.021.0219718
TCCTTTT507.2475814E-821.0219718
GGGTCTG250.003330596721.0219718
CAGTTTT950.021.0219718
ATCTTTT751.8189894E-1221.0219718
AGCTTTT1350.021.0219718
AAATTTT405.252481E-621.0219718
ACGTTTT850.021.0219718
GTTCTGT405.252481E-621.0219720
TTCTGTC651.200533E-1021.0219721
CAATTTT601.0095391E-921.0219718
GGTTCTG250.003330596721.0219719
CCGTTTT1250.021.0219718
TAGTTTT1000.021.0219718
ACATTTT405.252481E-621.0219718
ACTTTTT3050.021.0219719
AACTTTT800.021.0219718
GGCTTTT2650.021.0219718
TCGTTTT1000.021.0219718
GAATTTT1050.021.0219718
Produced by FastQC (version 0.11.2)