FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_60.3510000005f605.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_60.3510000005f605.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55474
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCTTACGCCTGTCTCTTATACAC2890.5209647762915961No Hit
GAATCCTTACGCTGTCTCTTATACACA640.11536936222374447No Hit
GAATCCTTACGCCCTGTCTCTTATACA580.1045534845152684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG405.2589803E-621.00000210
TCCTTTT651.1823431E-1021.00000218
ACGCCTA558.525603E-921.0000029
ACGCTTC558.525603E-921.0000029
ACGCCGT800.021.0000029
ACGCCCT651.1823431E-1021.0000029
ACGCCCG1100.021.0000029
ACGCCCA405.2589803E-621.0000029
ACGCTCT405.2589803E-621.0000029
ACGCCAT303.8689066E-421.0000029
ACGCTCC405.2589803E-621.0000029
ACGCTCA558.525603E-921.0000029
ACGCCAG800.021.0000029
ACGCCAC405.2589803E-621.0000029
ACGCTAT601.0059011E-921.0000029
CCAGTTT303.8689066E-421.00000217
ACGTTTT405.2589803E-621.00000218
CGCCCGT303.8689066E-421.00000210
GTCGTTT601.0059011E-921.00000217
CCTGTTT303.8689066E-421.00000217
Produced by FastQC (version 0.11.2)