FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_6.3510000005ef36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_6.3510000005ef36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13951
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATCGTAGCCTGTCTCTTATACACA1370.9820084581750411No Hit
CTGATCGTAGCACCTGTCTCTTATACA370.265213963156763No Hit
CTGATCGTAGCAGCTGTCTCTTATACA220.15769478890402122No Hit
CTGATCGTAGCAGGGGGGGGTTTTTTT190.13619095405347287No Hit
CTGATCGTAGCATCTGTCTCTTATACA150.10751917425274174No Hit
CTGATCGTAGCCTGTCTCTTCTACCCC140.10035122930255895No Hit
CTGATCGTAGCCTGTCTCTTCTACACA140.10035122930255895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGCT354.198747E-521.00000210
GGATTTT354.198747E-521.00000218
GATCGTA13050.021.0000023
GAGTTTT354.198747E-521.00000218
CGGGTTT354.198747E-521.00000217
AGCAGCG701.0913936E-1121.0000029
AGCAGAG354.198747E-521.0000029
GCAGTAC354.198747E-521.00000210
AGCATGG354.198747E-521.0000029
TGGTTTT250.003217455521.018
GGGTTTT1000.021.018
GGCATTT250.003217455521.017
AATTTTT506.312075E-821.019
TTTCTGT250.003217455521.021
GCAGGTT250.003217455521.010
GCAGGTG404.807687E-621.010
GTTCTGT250.003217455521.020
GGTCTGT303.6717884E-421.019
GCAGGGT303.6717884E-421.010
GCAGGGA250.003217455521.010
Produced by FastQC (version 0.11.2)