FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_58.3510000005f5b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_58.3510000005f5b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27310
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC1980.7250091541559869No Hit
ACGTCTCTGCCTGTCTCTTATACACAT1020.37348956426217506No Hit
ACGTCTCTGCATCTGTCTCTTCTACAC800.2929329915781765No Hit
ACGTCTCTGCCTGTCTCTTATCCACAT460.1684364701574515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCTC609.2040864E-1021.03671
ACGTCTC23400.021.0366971
ACGTTTC250.00326373721.0366971
ATGTCTC850.021.0366971
ACGTATC1950.021.0366951
CTCTGCA24900.020.9981695
ATGGGGG354.3979773E-520.99816911
TGCATTC1300.020.9981698
TGCATTA1400.020.9981698
GTGTTTT1100.020.99816918
GGGTTTT1250.020.99816918
TGCATGT2250.020.9981698
TGCATGG4350.020.9981698
CAGTTTT651.09139364E-1020.99816918
TGCATCC1500.020.9981698
TGCATCA1100.020.9981698
CATGAGC354.3979773E-520.99816910
TGCATAT1250.020.9981698
ACGTTTT354.3979773E-520.99816918
GGCGTTT455.934089E-720.99816917
Produced by FastQC (version 0.11.2)