FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_55.3510000005f557.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_55.3510000005f557.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58176
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC2590.44520077007700776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC507.1968316E-821.0163521
ATTTCTG354.4798697E-521.0163520
GGAGATT303.874187E-420.99828116
CTACAGC601.0095391E-920.9982811
TGGTTTT1850.020.99828118
ACAGCAA601.0095391E-920.9982813
CTTCAGC46350.020.9982811
TTCATTT303.874187E-420.99828117
TGCACGC303.874187E-420.99828110
ACGCTTT303.874187E-420.99828117
TGCATGA303.874187E-420.99828110
CAATGCT13150.020.9982817
CAATGCC11900.020.9982817
GTCATTT601.0095391E-920.99828117
AGCTTTT601.0095391E-920.99828118
TGCGTTG303.874187E-420.99828110
TACAGCA601.0095391E-920.9982812
TGCGTGA303.874187E-420.99828110
TGCGCAT303.874187E-420.99828110
TGCGTCC303.874187E-420.99828110
Produced by FastQC (version 0.11.2)