FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_52.3510000005f4f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_52.3510000005f4f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15727
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGTCGATACCTGTCTCTTATACAC1200.7630190118903796No Hit
AGACGTCGATACCTGTCTCTTCTACAC460.29249062122464553No Hit
AGACGTCGATACGGGGGGGGTTTTTTT200.12716983531506326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGC1500.021.0000028
GTCGTCG751.8189894E-1221.0000022
AGTCGTC751.8189894E-1221.0000021
TCGTCGA751.8189894E-1221.0000023
TTGTTTT354.2428677E-521.018
TGGTTTT659.822543E-1121.018
GTGTTTT404.8728252E-621.018
GCCTTTT250.00323548921.018
ATCTTTT250.00323548921.018
CGATACT3600.021.07
CGATACG6400.021.07
CGATACC3050.021.07
CGATACA2550.021.07
GATACTG1450.021.08
GATACTC850.021.08
GATACTA455.60216E-721.08
TCGATAC15600.021.06
AATTTTT659.822543E-1121.019
ACGTTTT354.2428677E-521.018
GATACGT1000.021.08
Produced by FastQC (version 0.11.2)