FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_50.3510000005f4c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_50.3510000005f4c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20926
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGTGAGCTAGGGCTGTCTCTTATA470.2246009748638058No Hit
GGTAGTGAGCTAGGGGCTGTCTCTTAT220.10513237121284526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT303.7584087E-421.00000218
CAGTTTT303.7584087E-421.00000218
GCTAGGG1100.021.0000029
GCTAGCT303.7584087E-421.0000029
AGCTATA609.0221874E-1021.0000028
AGCTAGG3600.021.0000028
AGCTAGC2200.021.0000028
TTATTTT455.791717E-721.00000218
AGCTACC609.0221874E-1021.0000028
AGCTAAT455.791717E-721.0000028
AGCTAAA1100.021.0000028
GTTTCTG303.7584087E-421.00000220
CATTTTT900.021.00000219
CTAGGTG303.7584087E-421.00000210
GCTACTG455.791717E-721.0000029
GCTATTG303.7584087E-421.0000029
ACTTTTT609.0221874E-1021.00000219
CTAGGGT455.791717E-721.00000210
CTAGGGA303.7584087E-421.00000210
GCTACGT303.7584087E-421.0000029
Produced by FastQC (version 0.11.2)