FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_5.3510000005ef1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_5.3510000005ef1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54093
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTACGTCTGTCTCTTATACACATC1630.30133288965300503No Hit
GAGCTACGTCTCTGTCTCTTATACACA600.11092008208086074No Hit
GAGCTACGTCTAGGGGCTGTCTCTTAT590.10907141404617973No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCAC354.468766E-521.0185181
GAACTAC303.844144E-421.0185181
GATCTAC1050.021.0185181
GAGATAC354.468766E-521.0185181
GTGCTAC2000.021.0185171
ATCTTTT558.518327E-920.99907718
TCTAGGG3000.020.9990779
TCTAGAT751.8189894E-1220.9990779
TCTAGAA751.8189894E-1220.9990779
TTATTTT751.8189894E-1220.99907718
CGCTTTT1500.020.99907718
TTCTGTC751.8189894E-1220.99907721
TCTACTT558.518327E-920.9990779
TCTACTC558.518327E-920.9990779
TCTATTT751.8189894E-1220.9990779
TCTATGC751.8189894E-1220.9990779
GAGTTTT1100.020.99907718
TCTATAG751.8189894E-1220.9990779
CTAGGGT558.518327E-920.99907710
CTAGGGG1500.020.99907710
Produced by FastQC (version 0.11.2)