FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_49.3510000005f4a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_49.3510000005f4a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43040
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCTGCTCCTGTCTCTTATACACAT1070.24860594795539032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAGG456.0507955E-721.016310
TCATGTT456.0507955E-721.01639
TCATGTA900.021.01639
CATGGGT456.0507955E-721.016310
CATGGGC456.0507955E-721.016310
CATGGAG456.0507955E-721.016310
TCATCGT456.0507955E-721.01639
TCATTGG1250.021.01639
TCATTCG456.0507955E-721.01639
GCTCATG17000.021.01637
TCATATA456.0507955E-721.01639
TCATAGG1250.021.01639
CATGCGG456.0507955E-721.016310
ATGGGGG405.17845E-621.01629811
ATGGGGA250.003312860621.01629811
CATTGTG303.8291654E-421.01629810
CATTGTA250.003312860621.01629810
CATGAGC303.8291654E-421.01629810
CATGAGA405.17845E-621.01629810
CATGACG354.4449705E-521.01629810
Produced by FastQC (version 0.11.2)