FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_44.3510000005f3f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_44.3510000005f3f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34289
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT354.437555E-521.00000218
CTCTGCT1200.021.0000028
CTCTGCC1050.021.0000028
CTGAGTG303.8272492E-421.00000210
GGCATTT303.8272492E-421.00000217
CTGAGGG354.437555E-521.00000210
CTGGGGG850.021.00000210
TCTGGTA701.2732926E-1121.0000029
CTGGGCT354.437555E-521.00000210
CTGGGCG303.8272492E-421.00000210
CTGGGCA354.437555E-521.00000210
TCTGGGA850.021.0000029
TCTGGCT701.2732926E-1121.0000029
TCTGGCC609.658834E-1021.0000029
TCTGGAC850.021.0000029
TCTGGAA303.8272492E-421.0000029
CGCTTTT303.8272492E-421.00000218
CAATTTT303.8272492E-421.00000218
CCGTTTT354.437555E-521.00000218
GCTATTT303.8272492E-421.00000217
Produced by FastQC (version 0.11.2)