FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_42.3510000005f3b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_42.3510000005f3b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51856
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCCT651.1823431E-1021.0000029
ACTGTAA651.1823431E-1021.0000029
ACTGACG651.1823431E-1021.0000029
CTGGGCA651.1823431E-1021.00000210
AGTGTTT651.1823431E-1021.00000217
GCGTTTT1300.021.00000218
GACTGCA2600.021.0000028
AGACTGC10150.021.0000027
GGTGTTT651.1823431E-1021.00000217
ACTGCTT405.24807E-621.09
ACTGCTG850.021.09
ACTGCTC354.49477E-521.09
ACTGCTA507.218114E-821.09
ACTGTTG1050.021.09
ACTGTTC751.8189894E-1221.09
ACTGTTA601.0004442E-921.09
TCCTTTT354.49477E-521.018
TGTTGGG250.003337026321.011
ACTGCGT751.8189894E-1221.09
ACTGCGC601.0004442E-921.09
Produced by FastQC (version 0.11.2)