FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_40.3510000005f372.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_40.3510000005f372.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47776
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTTGACCTGTCTCTTATACAC2020.42280643000669793No Hit
GTTGAGCTTGACCTGTCTCTTCTACAC560.11721366376423309No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTT303.858021E-421.00000216
GAGCGTT303.858021E-421.00000216
GGGTCTG405.2336654E-621.00000218
GTCATTT303.858021E-421.00000217
GGCATTT303.858021E-421.00000217
CGGAGGG303.858021E-421.00000212
TGCGTTT303.858021E-421.00000217
TGACCTC651.1823431E-1021.0000029
TGACCTA405.2336654E-621.0000029
ACTGAGG303.858021E-421.00000211
CGGGGTT405.2336654E-621.00000216
CGGGGTG303.858021E-421.00000212
TGACCCT609.949872E-1021.0000029
TGACCCG303.858021E-421.0000029
TGACCAT405.2336654E-621.0000029
GACAGGT609.949872E-1021.00000210
GACAGGC303.858021E-421.00000210
TGACTAC303.858021E-421.0000029
TTATTTT609.949872E-1021.00000218
GCGGTTT651.1823431E-1021.00000217
Produced by FastQC (version 0.11.2)