FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_39.3510000005f359.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_39.3510000005f359.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32905
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCGTCAGCCTGTCTCTTATACACA350.10636681355417109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCC950.021.0000028
CAGTTTT456.0066486E-721.00000218
AGCTCTT303.8227983E-421.0000029
AGCTCTC456.0066486E-721.0000029
AGCTTTG609.622454E-1021.0000029
AGCTCGT456.0066486E-721.0000029
AGCTCGG900.021.0000029
AGCTTGT950.021.0000029
AGCTTGA609.622454E-1021.0000029
AGCTCCG303.8227983E-421.0000029
AGCTCAT303.8227983E-421.0000029
AGCTTAT609.622454E-1021.0000029
AGCTTAA303.8227983E-421.0000029
AAATTTT303.8227983E-421.00000218
GCTAGGA303.8227983E-421.00000210
GGCGTTT609.622454E-1021.00000217
AGCTATC303.8227983E-421.0000029
AGCTAGT609.622454E-1021.0000029
AGCTAGC456.0066486E-721.0000029
AGCTACG303.8227983E-421.0000029
Produced by FastQC (version 0.11.2)