FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_38.3510000005f34c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_38.3510000005f34c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40277
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGCCGAACCTGTCTCTTATACACA880.21848697767956898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT1300.021.00000218
GCCTTTT507.11716E-821.00000218
AACAACA250.003324194321.0000029
GAAATTT250.003324194321.00000217
ACAGAGT250.003324194321.00000210
ACATGGT250.003324194321.00000210
TCCTATT250.003324194321.00000216
ACATGAG250.003324194321.00000210
TTTCTGT507.11716E-821.00000221
CCGCTTT250.003324194321.00000217
TGATCTG250.003324194321.00000218
GTTCCCG507.11716E-821.0000022
GATGCCG651.1459633E-1021.0000022
ACACCTT250.003324194321.00000210
TGTTCCC507.11716E-821.0000021
GGTTCTG250.003324194321.00000219
TGCTGCC651.1459633E-1021.0000021
ACACTGA250.003324194321.00000210
CAATTGG250.003324194321.00000211
TCAATTT250.003324194321.00000217
Produced by FastQC (version 0.11.2)