FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_36.3510000005f308.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_36.3510000005f308.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25533
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA1210.47389652606430893No Hit
CAGAACGACAGTGGCTGTCTCTTATAC330.1292445071084479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACG651.05501385E-1021.0392931
CTGAACG506.725713E-821.0392931
CAGACCG800.021.0392931
CAGTCGT303.792542E-420.9980419
CAGTTCT303.792542E-420.9980419
CAGTTCG303.792542E-420.9980419
CAGTTAT303.792542E-420.9980419
CAGTTAG303.792542E-420.9980419
AACGACA23900.020.9980414
CAGTAGT303.792542E-420.9980419
CAGTAGC303.792542E-420.9980419
AGTGGTA303.792542E-420.99804110
TTCTGTC609.331416E-1020.99804121
CATTTTT609.331416E-1020.99804119
GTGGGGT303.792542E-420.99804111
AGTGTGG303.792542E-420.99804110
CCTTTTT609.331416E-1020.99804119
GGCTTTT303.792542E-420.99804118
AGTGAGG303.792542E-420.99804110
TGGGCTG303.792542E-420.99804117
Produced by FastQC (version 0.11.2)