FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_35.3510000005f2ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_35.3510000005f2ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47316
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA3730.7883168484233662No Hit
CACAGAAGGACTGGGGGCTGTCTCTTA590.12469354975061289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTG354.484014E-521.00000210
ACTGCTA303.8572762E-421.00000210
ACTGTTG354.484014E-521.00000210
ACTGCGA250.00333273921.00000210
ACTGTGC250.00333273921.00000210
ACTGCCC250.00333273921.00000210
ACTGTCG303.8572762E-421.00000210
ACTGCAG303.8572762E-421.00000210
ACTGCAC250.00333273921.00000210
ACTGCAA250.00333273921.00000210
ACTGTAG303.8572762E-421.00000210
ACTCGTA250.00333273921.00000210
ACAAAAG303.8572762E-421.0000022
GCCTTTT250.00333273921.00000218
ACTCGGG701.4551915E-1121.00000210
ATCTTTT354.484014E-521.00000218
ACTCGAG303.8572762E-421.00000210
ACTCGAA303.8572762E-421.00000210
CTGAGGG303.8572762E-421.00000211
CTTTAGG303.8572762E-421.00000211
Produced by FastQC (version 0.11.2)