FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_34.3510000005f2c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_34.3510000005f2c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28692
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGTCTCGATCTGTCTCTTATACAC1690.589014359403318No Hit
ACTCGTCTCGATCTGTCTCTTCTACAC620.2160881081834658No Hit
ACTCGTCTCGATGCTGTCTCTTATACA390.13592639063153492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCA1500.021.0000028
CTCGATT6000.021.0000027
CTCGATG11000.021.0000027
CGATTAG751.8189894E-1221.0000029
GATGGGT303.8060837E-421.010
GATGGGA354.404872E-521.010
TTGTTTT900.021.018
GATGGCA303.8060837E-421.010
GATGGAG405.113514E-621.010
ACACGTC303.8060837E-421.01
ATGGGGG405.113514E-621.011
ATGGGGA303.8060837E-421.011
GAGCGTT250.003301062221.016
GGTTGTT250.003301062221.016
ACTCGTC25000.021.01
GTGTTTT609.458745E-1021.018
GCCTTTT354.404872E-521.018
CAGTTTT303.8060837E-421.018
CCTATTT250.003301062221.017
CGATATT506.937262E-821.09
Produced by FastQC (version 0.11.2)