FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_33.3510000005f2aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_33.3510000005f2aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34613
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGGAATGGCCTGTCTCTTATACACA840.24268338485540114No Hit
AACGGAATGGCTGCTGTCTCTTATACA390.11267442868286481No Hit
AACGGAATGGCTGTCTCTTATACACAT370.10689625285297431No Hit
AACGGAATGGCTCTGTCTCTTATACAC360.10400716493802907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTTT405.1648894E-621.00000218
TGATTTT558.249117E-921.00000218
GATGTTT405.1648894E-621.00000217
AGGAATG405.1648894E-621.0000023
CGGATTT405.1648894E-621.00000217
GGCTGGC1100.021.0000029
GGCTGCC405.1648894E-621.0000029
GGCTGCA558.249117E-921.0000029
GGCTGAG1100.021.0000029
TAATTTT558.249117E-921.00000218
GGCTCGG800.021.0000029
GGCTTGT405.1648894E-621.0000029
AAAGGAA405.1648894E-621.0000021
GCTGAGG405.1648894E-621.00000210
AGGTTTT1100.021.00000218
GGCTACG558.249117E-921.0000029
AAGGAAT405.1648894E-621.0000022
AGATTTT558.249117E-921.00000218
GACTTTT558.249117E-921.00000218
GGAGTTT405.1648894E-621.00000217
Produced by FastQC (version 0.11.2)