FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_32.3510000005f280.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_32.3510000005f280.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41921
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC1970.4699315378927029No Hit
GGATAGTTAGCCTGTCTCTTATACACA700.16698074950502134No Hit
GGATAGTTAGCCCTGTCTCTTCTACAC660.1574389923904487No Hit
GGATAGTTAGCCCCTGTCTCTTATACA420.10018844970301281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCTG558.272764E-921.02270120
GTTCTGT354.431042E-521.022720
ATTCTGT354.431042E-521.022720
ATTTCTG354.431042E-521.022720
AGCCCGT507.143353E-820.9976149
AGCCCGG1200.020.9976149
AGCCCGC651.1641532E-1020.9976149
AGCCCGA800.020.9976149
AGCCTGG1200.020.9976149
AGCCTGA609.858923E-1020.9976149
TGGTTTT1300.020.99761418
AGCCCCT609.858923E-1020.9976149
AGCCCCG507.143353E-820.9976149
AGCCCCA250.003328643520.9976149
AGCCTCT303.8500546E-420.9976149
AGCCTCG558.38736E-920.9976149
AGCCCAT354.472175E-520.9976149
AGCCTCA456.095379E-720.9976149
AGCCCAG507.143353E-820.9976149
AGCCCAA303.8500546E-420.9976149
Produced by FastQC (version 0.11.2)