FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_31.3510000005f266.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_31.3510000005f266.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47973
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAAAT2000.021.0208781
CATACAT1100.021.0208781
CTGACAT1150.021.0208761
CAGACAT41950.021.0208761
TGGTTTT1300.020.99895918
GTATGTT250.003334361120.99895916
CAAGATT303.8596423E-420.99895910
CAAGATG250.003334361120.99895910
TCCTTTT507.1933755E-820.99895918
CAATGTA303.8596423E-420.99895910
CAAGAGT250.003334361120.99895910
CAAGAGG405.236676E-620.99895910
CAAGAGC250.003334361120.99895910
CAATGAC250.003334361120.99895910
GGCATTT405.236676E-620.99895917
ATCTTTT405.236676E-620.99895918
GCAAGTT950.020.9989599
GCAAGTG1000.020.9989599
GCAAGTA950.020.9989599
GCAAGGC800.020.9989599
Produced by FastQC (version 0.11.2)