FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_30.3510000005f24d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_30.3510000005f24d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25796
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC1050.4070398511397116No Hit
AGTGTGACGAACCTGTCTCTTCTACAC450.17444565048844782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTTT455.822658E-721.02918418
AGAGTGA455.822658E-721.0291841
AGTGTGA23150.021.0291841
GTCTTTT455.822658E-721.02918418
GGGATTT455.822658E-721.02918417
GGGGTTT455.822658E-721.02918417
TCTTTTT900.021.02918419
GACTTTT455.822658E-721.02918418
TTGTTTT506.7666406E-821.02918218
TGGTTTT850.021.02918218
TGCATTT250.003265476321.02918217
CAGTTTT651.07320375E-1021.02918218
AATTTTT651.07320375E-1021.02918219
GGCGTTT405.0187027E-621.02918217
TTTCTGT557.872586E-921.02918221
GGTCTTT303.7558828E-421.02918217
GCGGTTT354.3353746E-521.02918217
AGGGCTG250.003265476321.02918217
ATGGTTT303.7558828E-421.02918217
AGGGTTT250.003265476321.02918217
Produced by FastQC (version 0.11.2)