FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_3.3510000005eed7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_3.3510000005eed7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20860
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGAGTCTGTCTCTTATACAC2381.1409395973154361No Hit
GAGGAACAGAGTCTGTCTCTTCTACAC1120.5369127516778524No Hit
GAGGAACAGAGCTGTCTCTTATACACA640.30680728667305845No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT250.003234268621.04086718
GTGTTTT250.003234268621.04086718
CAGTTTT404.918649E-621.04086718
AGCTTTT250.003234268621.04086718
AATTTTT506.571099E-821.04086719
TGATTTT250.003234268621.04086718
TGTTTTT950.021.04086719
GTTTTTT4650.021.04086720
TTCTTTT250.003234268621.04086718
GTCTTTT404.918649E-621.04086718
CCTTTTT506.571099E-821.04086719
ATGTTTT250.003234268621.04086718
AGGTTTT404.918649E-621.04086718
CGGTTTT250.003234268621.04086718
TCATTTT250.003234268621.04086718
TCTTTTT800.021.04086719
ATTTTTT2850.021.04086720
AGTTTTT800.021.04086719
CTTTTTT2900.021.04086720
CGTTTTT800.021.04086719
Produced by FastQC (version 0.11.2)