FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_29.3510000005f223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_29.3510000005f223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48963
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTCCAAGCCTGTCTCTTATACACA3150.6433429324183567No Hit
AAGGTCCAAGCACTGTCTCTTATACAC3030.6188346302309907No Hit
AAGGTCCAAGCACTGTCTCTTCTACAC1480.30226906031084694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT950.021.00000218
GCCTTTT558.463758E-921.00000218
GGCATTT405.2381274E-621.00000217
AGCTTTT558.463758E-921.00000218
GATATTT405.2381274E-621.00000217
GCAGGGC558.463758E-921.00000210
GCAGGGA405.2381274E-621.00000210
GCAGGAA405.2381274E-621.00000210
GGATTTT800.021.00000218
CCGTTTT405.2381274E-621.00000218
TAGTTTT800.021.00000218
GAGTTTT800.021.00000218
GCAACGA405.2381274E-621.00000210
TCTGTTT405.2381274E-621.00000217
GCAGTGG800.021.00000210
GCAGCCG405.2381274E-621.00000210
GCAAAGG405.2381274E-621.00000210
GCACGGT405.2381274E-621.00000210
GTCTTTT558.463758E-921.00000218
AGGTTTT558.463758E-921.00000218
Produced by FastQC (version 0.11.2)