FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_26.3510000005f1b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_26.3510000005f1b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45341
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTGTGTCCTGTCTCTTATACAC4070.897642310491608No Hit
ACTGTCTCTGTCTCTTATACACATCTC3520.7763392955603097No Hit
ACTGTCTGTGTCCTGTCTCTTCTACAC1370.3021547826470523No Hit
ACTGTCTCTGTCTCTTATACCCATCTC560.12350852429368563No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCTG354.420572E-521.03539820
TTTCTGT405.1477637E-621.03539721
CAGTTTT507.1231625E-821.01215618
GGCATTT250.003319372221.01215617
AGCTTTT250.003319372221.01215618
AAATTTT558.365532E-921.01215618
CGCCTTT250.003319372221.01215617
ACGTTTT250.003319372221.01215618
CCTGTTT250.003319372221.01215617
GGCGTTT558.365532E-921.01215617
GTACTTT250.003319372221.01215617
TGATTTT850.021.01215618
CTCTTTT507.1231625E-821.01215618
CGCTTTT558.365532E-921.01215618
ATGGTTT250.003319372221.01215617
GGATTTT1100.021.01215618
CAATTTT507.1231625E-821.01215618
CCGTCTG1100.021.0121562
CCGTTTT507.1231625E-821.01215618
TAGTTTT900.021.01215618
Produced by FastQC (version 0.11.2)