FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_24.3510000005f174.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_24.3510000005f174.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29680
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGAGGCTGTCTCTTATAC2420.8153638814016172No Hit
GTGTAGTGTGGCTGTCTCTTATACACA1990.6704851752021563No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC900.3032345013477089No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC810.272911051212938No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC740.24932614555256064No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC720.24258760107816713No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC640.215633423180593No Hit
GTGTAGTGTGGACCTGTCTCTTATACA610.2055256064690027No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC480.16172506738544476No Hit
GTGTAGTGTGGAGGGGCTGTCTCTTAT410.1381401617250674No Hit
GTGTAGTGTGGACCCTGTCTCTTATAC370.12466307277628032No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA350.1179245283018868No Hit
GTGTAGTGTGGAATCTGTCTCTTATAC320.1078167115902965No Hit
GTGTAGTGTGGAGGGGGGGGTTTTTTT310.10444743935309973No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGATG250.00330376321.00000210
GGATGCG354.4115965E-521.00000210
CAGTTTT558.127245E-921.00000218
GGCATTT354.4115965E-521.00000217
GGGCATT250.00330376321.00000216
AGTAGTG850.021.0000022
GGCGTTT250.00330376321.00000217
TTATTTT250.00330376321.00000218
TGATTTT354.4115965E-521.00000218
GTATTTT250.00330376321.00000218
GGTTCTG250.00330376321.00000219
CCGTTTT250.00330376321.00000218
TAGTTTT354.4115965E-521.00000218
CTGGTTT250.00330376321.00000217
TGCCTTT250.00330376321.00000217
GCAGTTT250.00330376321.00000217
GCTGTTT250.00330376321.00000217
AAGGTTT250.00330376321.00000217
TGGACTT250.00330376321.0000029
TGGACTA250.00330376321.0000029
Produced by FastQC (version 0.11.2)