FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_23.3510000005f15b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_23.3510000005f15b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41789
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA1620.387661824882146No Hit
ATCTGCTATCCGGGGGGGGGTTTTTTT430.10289789178970542No Hit
ATCTGCTATCCTGTCTCTTATACACAT420.10050491756203786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGCT800.021.0444181
ATCTACT701.2732926E-1121.0444181
ACCTGCT558.1709E-921.0444181
AGCTGCT2300.021.0444181
ATATGCT506.974733E-821.0444161
ATCTGCT35400.021.0444161
CCTGCTA558.285497E-921.0191862
TTTCTGT558.285497E-921.01918621
TCTGCTA35350.021.0191862
TCTCCTA303.8230256E-421.0191862
ACTGCTA800.021.0191842
TTCTGTC900.021.01918421
TCTACTA701.2732926E-1121.0191842
GCTGCTA2300.021.0191842
ATCTGTC456.034061E-721.01918420
TTTGCTA1000.021.0191842
TATCCGT8650.020.9940157
GTCATTT303.8541958E-420.99401317
GGCATTT303.8541958E-420.99401317
CCGGAGG609.877112E-1020.99401310
Produced by FastQC (version 0.11.2)