FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_22.3510000005f131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_22.3510000005f131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49289
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGAACTACCGCTGTCTCTTATACA1130.22926007831361966No Hit
GTCAGAACTACCCCTGTCTCTTATACA990.2008561748057376No Hit
GTCAGAACTACCGGGGGGGGTTTTTTT660.1339041165371584No Hit
GTCAGAACTACCTCTGTCTCTTATACA560.11361561403152834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT1900.021.01626218
GGCATTT456.0881575E-721.01626217
ACGTTTT405.204025E-621.01626218
GGCGTTT405.204025E-621.01626217
TAAGTTT405.204025E-621.01626217
CGCTTTT405.204025E-621.01626218
GAGTTTT1150.021.01626218
TCTGTTT405.204025E-621.01626217
GCGCTTT405.204025E-621.01626217
GGCTTTT950.021.01626218
GCGCTGT405.204025E-621.01626218
TAATTTT456.0881575E-721.01626218
GCGTTTT900.021.01626218
GTGATTT405.204025E-621.01626217
ATGTTTT900.021.01626218
TTGGTTT456.0881575E-721.01626217
GTGGTTT900.021.01626217
GCTTTTT2700.021.01626219
TGTGTTT456.0881575E-721.01626217
TCCTTTT250.003319695421.0162618
Produced by FastQC (version 0.11.2)