FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_17.3510000005f0ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_17.3510000005f0ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12635
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTGCGTATGCTGTCTCTTATACAC660.5223585278986942No Hit
AACGTGCGTATGGCTGTCTCTTATACA310.2453502176493866No Hit
AACGTGCGTATGGGCTGTCTCTTATAC280.221606648199446No Hit
AACGTGCGTATCTGTCTCTTATACACA260.20577760189948555No Hit
AACGTGCGTATGCTGTCTCTTCTACAC210.16620498614958448No Hit
AACGTGCGTATGCCTGTCTCTTATACA180.14246141669964385No Hit
AACGTGCGTATGAGCTGTCTCTTATAC150.1187178472497032No Hit
AACGTGCGTATGACCTGTCTCTTATAC150.1187178472497032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGTC354.1566815E-521.0000028
ATGGGGG354.1566815E-521.00000210
GTATGCC354.1566815E-521.0000028
GGGTTTT701.0913936E-1121.00000218
GTATGAT701.0913936E-1121.0000028
ATGCGGG354.1566815E-521.00000210
TATGAAG354.1566815E-521.0000029
GAGTTTT354.1566815E-521.00000218
AGGTTTT354.1566815E-521.00000218
GGGGTTT354.1566815E-521.00000217
TATGGTC354.1566815E-521.0000029
TATGGGC354.1566815E-521.0000029
GTATGTT404.7457415E-621.08
GTATGTG950.021.08
GTATGTA455.419806E-721.08
GTATGGT950.021.08
GTATGGG1600.021.08
GTATGGC950.021.08
GTATGGA1000.021.08
AACGTGC11800.021.01
Produced by FastQC (version 0.11.2)