FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_16.3510000005f082.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_16.3510000005f082.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40342
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACACCATAGCCTGTCTCTTATACAC2910.7213326062168459No Hit
GAACACCATAGCGGCTGTCTCTTATAC1790.4437063110406029No Hit
GAACACCATAGCCTGTCTCTTCTACAC1230.3048931634524813No Hit
GAACACCATAGCGCCTGTCTCTTATAC1060.26275345793465865No Hit
GAACACCATAGCAGCTGTCTCTTATAC1030.25531703931386646No Hit
GAACACCATAGCCCCTGTCTCTTATAC970.24044420207228198No Hit
GAACACCATAGCGTCTGTCTCTTATAC950.2354865896584205No Hit
GAACACCATAGCTGTCTCTTATACACA740.18343165931287492No Hit
GAACACCATAGCACCTGTCTCTTATAC730.18095285310594417No Hit
GAACACCATAGCTGCTGTCTCTTATAC610.15120717862277527No Hit
GAACACCATAGCATCTGTCTCTTATAC600.14872837241584452No Hit
GAACACCATAGCCGCTGTCTCTTATAC590.1462495662089138No Hit
GAACACCATAGCGACTGTCTCTTATAC500.1239403103465371No Hit
GAACACCATAGCTCCTGTCTCTTATAC420.10410986069109118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCA609.822543E-1021.0000029
TAGCGAC303.843566E-421.0000029
TAGCGAA950.021.0000029
CAGTTTT609.822543E-1021.00000218
AGCTCGG303.843566E-421.00000210
AGCTTGT303.843566E-421.00000210
AGCTTAG303.843566E-421.00000210
AAATTTT303.843566E-421.00000218
AGGATTT303.843566E-421.00000217
ACCATAG38550.021.0000025
AGCTAGT303.843566E-421.00000210
TAGCCTT558.352799E-921.0000029
TAGCCTA558.352799E-921.0000029
TAGCTTA558.352799E-921.0000029
TAGCCGC558.352799E-921.0000029
TAGCTGC609.822543E-1021.0000029
TAGCCCC303.843566E-421.0000029
TAGCCCA558.352799E-921.0000029
TAGCTCA303.843566E-421.0000029
CGGATTT303.843566E-421.00000217
Produced by FastQC (version 0.11.2)