FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_14.3510000005f04f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_14.3510000005f04f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24152
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTCTGTTGCTGTCTCTTATACACA2150.8901954289499834No Hit
ATGCTCTGTTGCGGCTGTCTCTTATAC540.22358396820139118No Hit
ATGCTCTGTTGCGGGGCTGTCTCTTAT530.21944352434580985No Hit
ATGCTCTGTTGCCTGTCTCTTATACAC420.17389864193441537No Hit
ATGCTCTGTTGCGCCTGTCTCTTATAC290.12007287181185823No Hit
ATGCTCTGTTGCGGGGGGGGTTTTTTT270.11179198410069559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTTT3800.021.0394220
ATACACA455.764268E-721.0394221
TATACAC506.6875145E-821.03941720
TTTCTGT404.9760692E-621.03941721
GTTCTGT303.734152E-421.03941720
TTCTGTC557.767085E-921.03941721
GTTTCTG250.003250427321.03941720
TGTTGCA3150.020.9958617
GTTGCGT2150.020.9958618
GTTGCCG2150.020.9958618
TGTTGCG11450.020.995867
TGTTGCC4750.020.995867
GTGTTTT455.881411E-720.9958618
TTGCAGA405.066964E-620.995869
AATTTTT900.020.9958619
TGCGCGG354.3745E-520.9958610
TGCGTGG405.066964E-620.9958610
TGCTGTG354.3745E-520.9958610
TGCGAGA354.3745E-520.9958610
TGCTGGG455.881411E-720.9958610
Produced by FastQC (version 0.11.2)