FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_13.3510000005f025.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_13.3510000005f025.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16781
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTATGGTGGCTGTCTCTTATACA960.5720755616471009No Hit
GACTCTATGGTCTGTCTCTTATACACA830.4946069960073893No Hit
GACTCTATGGTGCCTGTCTCTTATACA540.32179250342649424No Hit
GACTCTATGGTGTCTGTCTCTTATACA460.27411953995590255No Hit
GACTCTATGGTGACTGTCTCTTATACA280.1668553721470711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT303.7151406E-421.00000218
TGGTTTT303.7151406E-421.00000218
GGTGAGG455.6494173E-721.0000029
GGTGAGC455.6494173E-721.0000029
GGTGAGA303.7151406E-421.0000029
CTATATG900.021.0000023
CTCTATG15150.021.0000023
GGCTTTT303.7151406E-421.00000218
GCGTTTT303.7151406E-421.00000218
ATATGGT900.021.0000025
AGGTTTT303.7151406E-421.00000218
TGGGTTT303.7151406E-421.00000217
GGTGGTG557.503331E-921.0000029
GGTGGCG455.6494173E-721.0000029
GGTGGCC557.503331E-921.0000029
CGGTTTT303.7151406E-421.00000218
TGGTGCC900.021.0000028
TGGTGCA455.6494173E-721.0000028
GGTGCTT303.7151406E-421.0000029
GGTGCTA303.7151406E-421.0000029
Produced by FastQC (version 0.11.2)