FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate6_12.3510000005f00b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate6_12.3510000005f00b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34285
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCTTACGCCTGTCTCTTATACAC1650.48126002625054687No Hit
GAATCCTTACGCCTGTCTCTTCTACAC550.16042000875018228No Hit
GAATCCTTACGCTGTCTCTTATACACA380.11083564240921685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGC303.8272492E-421.00000211
ACGCCTA303.8272492E-421.0000029
ACGCTTA303.8272492E-421.0000029
ACGCCCT303.8272492E-421.0000029
ACGCCCA303.8272492E-421.0000029
ACGCTCA303.8272492E-421.0000029
ACGCCAC303.8272492E-421.0000029
ACGTTTT354.437555E-521.00000218
GTCGTTT354.437555E-521.00000217
CGCCCCG303.8272492E-421.00000210
ACGCATA354.437555E-521.0000029
ACGCAGC354.437555E-521.0000029
ACGCAAG354.437555E-521.0000029
GTTCTGT609.658834E-1021.00000220
TTATTTT354.437555E-521.00000218
CTCTTTT303.8272492E-421.00000218
AGGGTTT303.8272492E-421.00000217
GCGGTGG354.437555E-521.00000211
TGTTTCT303.8272492E-421.00000219
GTTTCTG850.021.00000220
Produced by FastQC (version 0.11.2)