FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate5_96.3510000005ee7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate5_96.3510000005ee7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45343
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCGTGGAACCTGTCTCTTATACAC5261.1600467547361224No Hit
TAAGCGTGGAACCTGTCTCTTCTACAC2060.4543148887369605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGAAC44750.021.0000026
TGGTTTT1450.021.018
TTCATTT250.003330584621.017
TGCATTT250.003330584621.017
ACCGGGC250.003330584621.011
AACGCGG456.1114224E-721.010
AACGCGC354.4786128E-521.010
AACGTGC354.4786128E-521.010
AACGTGA456.1114224E-721.010
AACGTCT250.003330584621.010
AACGTAT456.1114224E-721.010
AACCGTG405.223834E-621.010
CAGTTTT701.4551915E-1121.018
GTCATTT250.003330584621.017
GGCATTT405.223834E-621.017
AACAAGT303.8537887E-421.010
AACAAGG250.003330584621.010
ATCTTTT250.003330584621.018
TAAATTT250.003330584621.017
GGGCATT250.003330584621.016
Produced by FastQC (version 0.11.2)