FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate5_88.3510000005ed7b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate5_88.3510000005ed7b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51198
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTTGACCTGTCTCTTATACAC3490.6816672526270557No Hit
GTTGAGCTTGACCTGTCTCTTCTACAC1150.22461814914645103No Hit
GTTGAGCTTGACGCTGTCTCTTATACA770.1503964998632759No Hit
GTTGAGCTTGACCCTGTCTCTTATACA700.13672409078479628No Hit
GTTGAGCTTGACGGCTGTCTCTTATAC540.10547287003398571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTTT1050.021.03134318
TGCATTT354.4416687E-521.03134217
GTGTTTT1600.021.03134218
GCCTTTT405.178117E-621.03134218
CAGTTTT900.021.03134218
GGCATTT354.4416687E-521.03134217
AGCTTTT900.021.03134218
CACATTT250.003307602621.03134217
TATATTT250.003307602621.03134217
AAATTTT405.178117E-621.03134218
TGCGTTT354.4416687E-521.03134217
TATACAC405.178117E-621.03134221
GATATTT354.4416687E-521.03134217
AATTTTT2050.021.03134219
TGTCTTT250.003307602621.03134217
CCTGTTT354.4416687E-521.03134217
GGCGTTT900.021.03134217
GTACTTT507.0998794E-821.03134217
GGACTTT354.4416687E-521.03134217
TTTCTGT507.0998794E-821.03134221
Produced by FastQC (version 0.11.2)