FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate5_80.3510000005ec89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate5_80.3510000005ec89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65349
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC3290.5034507031477146No Hit
GGATAGTTAGCCTGTCTCTTATACACA1490.22800654944987683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTT303.863809E-421.01226218
TTCATTT303.863809E-421.01226217
TGCGTTT303.863809E-421.01226217
GGCGCTG303.863809E-421.01226217
GGTCTTT601.0077201E-921.01226217
GAAGTTT601.0077201E-921.01226217
GATGTTT303.863809E-421.01226217
GCAATTT303.863809E-421.01226217
ATAATTT303.863809E-421.01226217
CGGGTTT601.0077201E-921.01226217
TCAGTTT303.863809E-421.01226217
AGAGTTT303.863809E-421.01226217
ATGCTTT303.863809E-421.01226217
TAATTTT601.0077201E-921.01226218
AGGTTTT1200.021.01226218
ATCGTTT303.863809E-421.01226217
TGAATTT303.863809E-421.01226217
GGAGTTT1200.021.01226217
GAGATTT601.0077201E-921.01226217
AAGTTTT1200.021.01226218
Produced by FastQC (version 0.11.2)