FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate5_8.3510000005e382.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate5_8.3510000005e382.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85154
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTCATGCTGTCTCTTATACAC17452.049228456678488No Hit
ACAGTGCTCATGGGCTGTCTCTTATAC3550.4168917490663973No Hit
ACAGTGCTCATGGCTGTCTCTTATACA1660.19494093054935765No Hit
ACAGTGCTCATGGCCTGTCTCTTATAC1510.17732578622260844No Hit
ACAGTGCTCATGGTCTGTCTCTTATAC1270.14914155529980974No Hit
ACAGTGCTCATGTGCTGTCTCTTATAC1000.11743429551166122No Hit
ACAGTGCTCCTGTCTCTTATACACATC980.11508560960142801No Hit
ACAGTGCTCATGAGCTGTCTCTTATAC870.10216783709514528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTGT1100.021.0321
GTTCTGT751.8189894E-1221.0320
TTCTGTC1250.021.0321
GTTTCTG601.0113581E-921.0320
ATTCTGT405.248601E-621.0320
ATTTCTG456.1582614E-721.0320
TTTTTCT250.00332679921.02999921
TCTACAC651.200533E-1021.02999921
TTCTACA701.4551915E-1121.02999920
TCCTTTT456.193259E-721.01763718
TGCATTT507.291965E-821.01763717
GTCATTT507.291965E-821.01763717
ATCTTTT354.5093548E-521.01763718
TATATTT250.00333817721.01763717
TCGATTT250.00333817721.01763717
TTCGTTT405.2754604E-621.01763717
TTACTTT250.00333817721.01763717
TGACTTT250.00333817721.01763717
CGCCTTT250.00333817721.01763717
ACGTTTT800.021.01763718
Produced by FastQC (version 0.11.2)