FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate5_72.3510000005eb8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate5_72.3510000005eb8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40211
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGAGGCTGTCTCTTATAC4751.181268807042849No Hit
GTGTAGTGTGGCTGTCTCTTATACACA4341.0793066573823082No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC1940.4824550496132899No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC1820.452612469224839No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC1790.44515182412772625No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC1640.40784859864216255No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC1640.40784859864216255No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC1220.3033995672825843No Hit
GTGTAGTGTGGACCTGTCTCTTATACA1190.29593892218547163No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA1160.2884782770883589No Hit
GTGTAGTGTGGAATCTGTCTCTTATAC1070.26609634179702074No Hit
GTGTAGTGTGGATACTGTCTCTTATAC920.22879311631145705No Hit
GTGTAGTGTGGAACCTGTCTCTTATAC880.21884558951530678No Hit
GTGTAGTGTGGATCTGTCTCTTATACA840.20889806271915645No Hit
GTGTAGTGTGGATCCTGTCTCTTATAC740.18402924572878068No Hit
GTGTAGTGTGGAAACTGTCTCTTATAC530.13180473004899157No Hit
GTGTAGTGTGGACCCTGTCTCTTATAC530.13180473004899157No Hit
GTGTAGTGTGGATTCTGTCTCTTATAC520.129317848349954No Hit
GTGTAGTGTGGACTCTGTCTCTTATAC520.129317848349954No Hit
GTGTAGTGTGGACACTGTCTCTTATAC480.11937032155380368No Hit
GTGTAGTGTGGAGGGGCTGTCTCTTAT420.10444903135957823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT1400.021.01992618
GTGTTTT1350.021.01992618
CAGTTTT950.021.01992618
ATCTTTT354.4297543E-521.01992618
TGATTTT701.2732926E-1121.01992618
AGGGTTT354.4297543E-521.01992617
CAATTTT354.4297543E-521.01992618
CCGTTTT354.4297543E-521.01992618
GATTTTT2450.021.01992619
GCGTTTT701.2732926E-1121.01992618
GTGATTT354.4297543E-521.01992617
GGGGTTT1100.021.01992617
TCATTTT354.4297543E-521.01992618
GCATTTT751.8189894E-1221.01992618
ATACACA558.260031E-921.01992621
CGTTTTT2100.021.01992619
GGGTTTT2400.021.01992418
GCCTTTT250.003305824521.01992418
GGCATTT250.003305824521.01992417
AGCTTTT405.156782E-621.01992418
Produced by FastQC (version 0.11.2)