FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate5_71.3510000005eb60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate5_71.3510000005eb60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73178
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA4740.6477356582579464No Hit
ATCTGCTATCCTGTCTCTTATACACAT1780.2432425045778786No Hit
ATCTGCTATCCGGCTGTCTCTTATACA980.1339200306102927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTGT558.474672E-921.03493321
GTTTCTG303.8418217E-421.03493120
AACGTTT405.252343E-621.02053517
TCCTTTT405.252343E-621.02053518
GTGTTTT2500.021.02053518
ACGCTTT250.003331054721.02053517
GCCTTTT1050.021.02053518
CCTATTT303.85966E-421.02053517
GGCATTT751.8189894E-1221.02053517
ATCTTTT751.8189894E-1221.02053518
TATATTT303.85966E-421.02053517
TGACTTT250.003331054721.02053517
CCAGTTT303.85966E-421.02053517
GCGATTT751.8189894E-1221.02053517
ACGTTTT1450.021.02053518
ATGATTT250.003331054721.02053517
AGGATTT558.543793E-921.02053517
GGCGTTT601.0095391E-921.02053517
CTCTTTT850.021.02053518
CAATTTT1350.021.02053518
Produced by FastQC (version 0.11.2)