FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate5_63.3510000005ea55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate5_63.3510000005ea55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62843
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAGCTGCTGTCTCTTATACACA5910.9404388714733543No Hit
TGGCATAGCTGAGGCTGTCTCTTATAC1330.211638527759655No Hit
TGGCATAGCTGAGGGGCTGTCTCTTAT1060.16867431535731903No Hit
TGGCATAGCTGAGGGGGGGGTTTTTTT1000.15912671260124436No Hit
TGGCATAGCTGCTGTCTCTTCTACACA690.10979743169485862No Hit
TGGCATAGCTGAGCCTGTCTCTTATAC640.1018410960647964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTT2450.021.0127518
GCCTTTT850.021.0127518
GTCATTT354.493175E-521.0127517
CCAGTTT354.493175E-521.0127517
GCGATTT456.1536775E-721.0127517
GTCGTTT456.1536775E-721.0127517
ACATTTT456.1536775E-721.0127518
GCAGTTT354.493175E-521.0127517
GCTGTTT558.521965E-921.0127517
TCGTTTT900.021.0127518
TGGATTT456.1536775E-721.0127517
GAATTTT456.1536775E-721.0127518
CGAGTTT456.1536775E-721.0127517
GTGATTT456.1536775E-721.0127517
ATGTTTT701.4551915E-1121.0127518
ACCTTTT354.493175E-521.0127518
GTGGTTT701.4551915E-1121.0127517
CTGTTTT1400.021.0127518
CGCATTT354.493175E-521.0127517
CGCGTTT701.4551915E-1121.0127517
Produced by FastQC (version 0.11.2)