FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate5_56.3510000005e98c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate5_56.3510000005e98c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56329
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTCATGCTGTCTCTTATACAC11832.10016155088853No Hit
ACAGTGCTCATGCTGTCTCTTCTACAC4560.809529727138774No Hit
ACAGTGCTCATGGGCTGTCTCTTATAC2700.47932681212164246No Hit
ACAGTGCTCATGGCTGTCTCTTATACA1450.25741625095421544No Hit
ACAGTGCTCATGGCCTGTCTCTTATAC1380.2449892595288395No Hit
ACAGTGCTCATGGTCTGTCTCTTATAC1080.191730724848657No Hit
ACAGTGCTCATGAGCTGTCTCTTATAC900.1597756040405475No Hit
ACAGTGCTCCTGTCTCTTATACACATC860.15267446608318982No Hit
ACAGTGCTCATGTGCTGTCTCTTATAC720.127820483232438No Hit
ACAGTGCTCATGCCTGTCTCTTATACA590.10474178487102559No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTG1150.021.01155710
ATGGGTA303.8555215E-421.01155710
TGTGAGG250.003329877721.01155711
ATGGGGT1000.021.01155710
ATGGGCG507.203744E-821.01155710
ATGGGCC507.203744E-821.01155710
CATGATA507.203744E-821.0115579
ATGGGAA507.203744E-821.01155710
CATGAGC701.4551915E-1121.0115579
CATGAGA456.133596E-721.0115579
CATGACT507.203744E-821.0115579
CATGACG900.021.0115579
CATGACC303.8555215E-421.0115579
CATGAAC601.0004442E-921.0115579
CATGAAA303.8555215E-421.0115579
ATGACGT250.003329877721.01155710
TTTCTGT456.133596E-721.01155721
ATGATGG250.003329877721.01155710
TTTTTTC303.8555215E-421.01155721
ATGGCGC303.8555215E-421.01155710
Produced by FastQC (version 0.11.2)