FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate5_48.3510000005e870.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate5_48.3510000005e870.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67710
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCGTGGAACCTGTCTCTTATACAC12191.8003249150790133No Hit
TAAGCGTGGAACTGTCTCTTATACACA1250.18461084034854527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCTG354.4745415E-521.02810720
ATTTCTG250.003321568521.02810720
AACGTTA303.884838E-420.99704710
AACAATG303.884838E-420.99704710
AACGTCT303.884838E-420.99704710
AACGTAT303.884838E-420.99704710
AGCTTTT950.020.99704718
AACAACG303.884838E-420.99704710
TTCGTTT303.884838E-420.99704717
GTCGTTT303.884838E-420.99704717
AACGAAC303.884838E-420.99704710
AACTGCT303.884838E-420.99704710
CGGGGGA303.884838E-420.99704712
TATGTTT303.884838E-420.99704717
GAACGTG2400.020.9970479
GAACGTA1200.020.9970479
AGGGTTT601.0186341E-920.99704717
GAACGCC601.0186341E-920.9970479
CGGATTT303.884838E-420.99704717
GGTTTCT303.884838E-420.99704719
Produced by FastQC (version 0.11.2)