FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate5_40.3510000005e771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate5_40.3510000005e771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76405
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTTGACCTGTCTCTTATACAC6270.8206269223218376No Hit
GTTGAGCTTGACGCTGTCTCTTATACA1480.1937046004842615No Hit
GTTGAGCTTGACCCTGTCTCTTATACA1180.15444015444015444No Hit
GTTGAGCTTGACGGCTGTCTCTTATAC940.12302859760486878No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGTT250.003351374521.00000216
ACAGTCG250.003351374521.00000211
GGTTGTT250.003351374521.00000216
ACCGGCG250.003351374521.00000211
ATCTTTT507.332346E-821.00000218
TAAATTT250.003351374521.00000217
TAGTGTT250.003351374521.00000216
TCGATTT250.003351374521.00000217
GACAGTC507.332346E-821.00000210
ACTGAGA250.003351374521.00000211
TGACCCT558.654752E-921.0000029
TGACTCC507.332346E-821.0000029
TGACCAC507.332346E-821.0000029
GTGTATT250.003351374521.00000216
GGGTATT507.332346E-821.00000216
TGACTAT558.654752E-921.0000029
TGACTAA558.654752E-921.0000029
GACAGAT250.003351374521.00000210
GACAGAA250.003351374521.00000210
ACGTTCG250.003351374521.00000211
Produced by FastQC (version 0.11.2)