FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate5_32.3510000005e68f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate5_32.3510000005e68f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112856
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC6100.540511802651166No Hit
GGATAGTTAGCCTGTCTCTTATACACA2650.2348125044304246No Hit
GGATAGTTAGCCCCTGTCTCTTATACA1260.11164670021974905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTTT405.311176E-621.01286117
TGCATTT405.311176E-621.01286117
AATCTTT405.311176E-621.01286117
ATCTTTT1150.021.01286118
GAAATTT558.705683E-921.01286117
TTACTTT405.311176E-621.01286117
TGACTTT558.705683E-921.01286117
GCGATTT303.8872645E-421.01286117
TGTCTTT303.8872645E-421.01286117
GGCGTTT1350.021.01286117
TAAGTTT651.2187229E-1021.01286117
TCGGTTT800.021.01286117
GAAGTTT558.705683E-921.01286117
TTATTTT1600.021.01286118
TTTTTTC303.8872645E-421.01286121
ATGGTTT1050.021.01286117
AGGGTTT1300.021.01286117
GTTTCTG651.2187229E-1021.01286120
GACATTT303.8872645E-421.01286117
AACTTTT1150.021.01286118
Produced by FastQC (version 0.11.2)