FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_96.3510000005e283.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_96.3510000005e283.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58768
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCGTGGAACCTGTCTCTTATACAC18893.214334331609039No Hit
TAAGCGTGGAACTGTCTCTTATACACA1670.2841682548325619No Hit
TAAGCGTGGAACGGGGGCTGTCTCTTA690.11741083582902259No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTGT2300.021.02898621
TTTTCTG405.2050127E-621.02898621
TTTTTCT354.460095E-521.02898621
GTTTCTG1050.021.02898620
ATTTCTG1000.021.02898620
CTTCTGT507.154085E-821.02898620
CTTTCTG250.003315576621.02898620
CGTTTCT303.8588909E-421.01107419
TTGTTTT601.0022632E-921.01107418
TGGTTTT601.0022632E-921.01107418
GGTTGTT250.003332032121.01107416
GCCTTTT456.1443825E-721.01107418
CAGTTTT701.4551915E-1121.01107418
GGCATTT456.1443825E-721.01107417
ATCTTTT405.243697E-621.01107418
AGCTTTT250.003332032121.01107418
GGGCATT250.003332032121.01107416
TGCGTTT250.003332032121.01107417
GATATTT456.1443825E-721.01107417
CCAGTTT250.003332032121.01107417
Produced by FastQC (version 0.11.2)