FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate4_93.3510000005e226.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate4_93.3510000005e226.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5396
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGACGCTGTCTCTTATACACATC80.14825796886582654No Hit
ATCTCGACGCTAGGCCTGTCTCTTATA60.1111934766493699No Hit
ATCTCGACGCTACCTGTCTCTTATACA60.1111934766493699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTT353.6017373E-521.00000218
AGCTCGA403.9442875E-621.0000021
GCTAGGG403.9442875E-621.0000029
TTTTTTT3200.021.00000221
CGCTATT353.6017373E-521.0000028
CGCTAGT353.6017373E-521.0000028
CGCTAGA353.6017373E-521.0000028
CGCTAAG403.9442875E-621.0000028
GCTCGAC403.9442875E-621.0000022
CTCGACG4700.021.03
ACGCTAT850.021.07
ACGCTAC1250.021.07
ACGCTAA1200.021.07
ATCTCGA4100.021.01
TCTAGAC605.347829E-1021.02
ATCTAGA605.347829E-1021.01
GTTTTTT1350.021.020
GGTTTTT655.638867E-1121.019
CGCTAGG850.021.08
CGCTAGC454.296744E-721.08
Produced by FastQC (version 0.11.2)